APPROCHE CHIMIO-MICROBIOLOGIQUE DE L’ACTIVITE DES HUILES ESSENTIELLES PRODUITES A L’INSTITUT MALGACHE DE RECHERCHES APPLIQUEES (IMRA)
Faculte De Medecine — nan — None ()
Auteur : RAKOTONIRIANA Erick Francisco Savigny Didier
Année de soutenance : 2004
Diplome : DEFS
Langue : FR
Résumé
Nowadays, aromatherapy is more and more prescribed to relieve or to treat many illnesses. The knowledge of their pharmacological properties, in particular their antimicrobial activity is more than necessary to establish a formulation and an adequate prescription. Disc diffusion method for the determination of the inhibition diameter and the microdilution method for the determination of the minimal inhibitory concentration (MIC) were performed to analysis the antimicrobial activity of five essential oils produced in “the Madagascar Applied Research Institute (IMRA): essential oils of Niaouli or Melaleuca quinquenervia var. viridiflora chemotype I, of cypress or Cupressus lusitanicus, of Cinnamomum camphora, of olive or Eucalyptus citriodora, and HE . These essential oils were assayed on 15 bacterial strains and 2 yeasts. In the order of decreasing activity, Eucalyptus citriodora showed a good antibacterial activity, with a broad spectrum of antibacterial and antifungal activity. Cupressus lusitanicus and HE were less active and their spectra especially concerned gram-positive bacteria. Melaleuca quinquenervia var. viridiflora chemotype I and Cinnamomum camphora exhibited a low antimicrobial activity. This antimicrobial activity was related to certain essential oil chemical constituents principally to citronnellal and 1-8 cineol. On the other hand, some minor constituents as α-terpineol possessed an inhibitory effect on this anti microbial activity